Journal: Virology

Article Title: Identification of key hemagglutinin residues responsible for cleavage, acid stability, and virulence of fifth-wave highly pathogenic avian influenza A(H7N9) viruses

doi: 10.1016/j.virol.2019.07.012

Figure Lengend Snippet: The TW/17 HA trimer was modeled with PyMOL version 2.2.3 from A/Guangdong/17SF003/2016 (PDB ID: 6D7U) with the highlighted residues HA1-(38, 112, 164, 217; H7 numbering), and HA2-64 in one monomer (left) and a close-up image of the residue HA2-K64 forming potential hydrogen bonds with HA2-N79 and the glycans of HA2-N82 from a neighboring monomer (right).

Article Snippet: At 3h post fusion induction, syncytia were visualized by immunofluorescence microscopy after staining the cells with rabbit anti -H7 HA monoclonal antibody (Sino Biological, Cat#11082-R002, PA) and Alexa Fluor™ 488-labeled anti-rabbit IgG secondary antibody (Thermo Fisher Scientific), and diamidino-2-phenylindole (DAPI) for nuclei.

Techniques:

Journal: Life Science Alliance

Article Title: Expression of TMPRSS2 is up-regulated by bacterial flagellin, LPS, and Pam3Cys in human airway cells

doi: 10.26508/lsa.202201813

Figure Lengend Snippet: (A) Calu-3 cells were treated with flagellin (200 ng/ml) for 24 h, then infected with H1N1pdm or H3N2 IAV at a low MOI and incubated for 48 h in the absence or presence of trypsin. Virus titers in supernatants were determined by the plaque assay at the indicated time points. Data shown are mean values + SD of four independent experiments. (B) Calu-3 cells stimulated with flagellin (fla) for 24 h as described above were inoculated with H1N1pdm at an MOI of 0.001 and incubated for 48 h with or without exogenous trypsin (try). Cell lysates were subjected to SDS–PAGE and Western blot analysis using H1N1-specific antibodies. β-actin served as a loading control. (C) Calu-3 cells were incubated with flagellin for 24 h and then infected with SARS-CoV-2 or SARS-CoV at a low MOI. Virus titers were determined by TCID 50 titration at indicated time points. Data are mean values + SD (n = 3). Statistics: a t test or Welch’s t test, ns P > 0.05 compared with the untreated infected control.

Article Snippet: A polyclonal rabbit serum against HA of H1N1 was obtained from Sino Biological (11085-T62).

Techniques: Infection, Incubation, Plaque Assay, SDS Page, Western Blot, Titration